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Image Search Results
Journal: Nucleic Acids Research
Article Title: The terminal loop region controls microRNA processing by Drosha and Dicer
doi: 10.1093/nar/gkq645
Figure Lengend Snippet: Interaction between Drosha and pri-miRNAs. 5′-end labeled, pri-miR-16, pri-miR-30 and pri-miR-31 substrates were digested with RNase A with or without prior incubation with Drosha. Sequences and the predicted secondary structures of the pri-miRNAs are shown on top. Stable RNase A cleavage products in the presence of Drosha were indicated with an asterisk. DNA size markers are indicated in the left. The pri-miR-30 substrate was incubated with two concentrations of Drosha (indicated as ‘1x’ and ‘2x’) before RNase A addition, and two RNA markers of 39-nt and 45-nt-long were run on the same gel for size comparisons: their 3′ ends are indicated by arrows on top. Shown are representatives of at least three independent experiments.
Article Snippet:
Techniques: Labeling, Incubation